Spectorophotometric

A Spectrophotometric Method for Measuring Serum Trypsin Inhibitor Capacity George M. Homer, Bernard J. Katchman, and Robert E. Zipf The spectrophotometric get of Schwert and Takenaka (11) for the assay of trypsinesterolytic activity hasbeen special for the measurementof the trypsin inhibitor capacityof blood serum. An averagevalue of 0.99 mg. of trypsin inhibited per milliliter of serum (3 S.D. = ±0.30) wasdetermined for 50 control subjects. affected role serum assayvaluesdeterminedwith this methodhavebeenfoundsimilarto thosedetermined with the circumscribed Kunitz method using l131-labeledcaseinas the substratein the normalrange for a pattern of pathologicconditionsand in the elevation range for abnormalities of the pancreas and generalized cancer conditions. From the standpointsof accuracy, simplicity, and speed this technic is believed to be an improvementover foregoingmethodsfor the measurementof this serum variable. lIHE NEED for additional blood tests for the di agnosis of pancreatic dysfunction, immix with our own recreate in diagnostic enzyme studies resulted in earlier work in this area (1, 2). A continued interest in the serum trypsin inhibitor values in cases of malignancy has farther ruttish the development of a less complex plainly more(prenominal) accurate procedure than that using 1131-laheled casein as the substrate.

introductory methods use for the measurement of this serum variable include the following(a): the use of jelly as a substrate, by Hussey and Northrop (3) ; a ref ractometric method using casein as a substrate, by G-rob (4); an assay gr ound on the dissolution of a blood clot, by ! Clark et al. (5) ; or hemostatic globulin in interpose of blood, by Christensen (6); fibrin tagged with J131as a substrate, by Shulman (7) ; and the spectroFrom the Department of Research, Miami valley hospital, Dayton, Ohio. Presented in part at the 13th Annual clashing of the American Association of Clinical Chemists, Aug. 31, 1961, refreshed York. Supported in part by grants from the...If you want to prolong a proficient essay, order it on our website: BestEssayCheap.com

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